|
| Status |
Public on Jun 29, 2007 |
| Title |
Pdr1-Cyc8 fusion Dpdr1, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast with deletion of endogenous Pdr1 gene and expressing Pdr1-Cyc8 fusion protein
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Yeast strain BY4741
|
| Growth protocol |
Yeast were grown in synthetic dextrose media, logarithmically growing, 30 degrees centigrade
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA using Ambion RiboPure-Yeast protocol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, revision 4, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array YG-s98. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from yeast containing deletion of endogenous Pdr1 gene and expressing Pdr1-Cyc8 fusion protein
|
| Data processing |
The data were analyzed with GeneChip Operating Software version 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jun 28, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
[email protected]
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE8326 |
Repression of pleiotrophic drug resistance genes in Saccharomyces cerevisiae using chimeric transcriptional repressors |
|
