|
| Status |
Public on Feb 18, 2016 |
| Title |
3s+ cells, IFNβ treated, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoma cells with floxed Runx1, treated with IFNβ
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/CBA
tissue: spleen
genotype/variation: Mx1Cre/Rx1fl/fl/EµMyc/p53+/-
cell name: 3s
treatment: IFNbeta
|
| Treatment protocol |
Established lymphoma lines from Mx1Cre+/Rx1fl/fl/EµMyc/p53+/- mice (3s) and MX1Cre+/Rx1WT/WT/EµMyc/p53+/- mice (30s) were treated with 50U/mL IFNβ or vehicle control (PBS+0.1% BSA) for 72 hrs, replated in fresh culture medium and grown for a further 6 days before harvesting.
|
| Growth protocol |
3s and 30s cell lines were cultured in RPMI 1640 with 10% FCS, 100U/ml Penicillin/streptomycin, 2mM L-glutamine and 50µM 2-mercaptoethanol at 37 degrees celcius, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by RNeasy kit (Qiagen) according to the manufacturer's instructions. Quality was assessed using an Agilent 2100 Bioanalyser and NanoDrop 2000 (Thermo Scientific)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared from 100ng total RNA (Applause WT-Amp ST Plus System, Manual M01199 v2 Nugen/Encore Biotin Module, Manual M0111 v5 Nugen)
|
| |
|
| Hybridization protocol |
3730 ng fragmented cDNA were hybridised for 18 hr at 45 degrees celcius on GeneChip MTA 1.0 Arrays, then GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneChip scanner 3000 7G.
|
| Description |
Gene expression data from IFNβ treated lymphoma cells with floxed Runx1
|
| Data processing |
The data were analyzed on Gene Level with the RMA algorithm of the Expression Console 1.4.
|
| |
|
| Submission date |
Feb 17, 2016 |
| Last update date |
Feb 18, 2016 |
| Contact name |
Kathryn Gilroy |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Glasgow
|
| Street address |
Garscube Estate
|
| City |
Glasgow |
| ZIP/Postal code |
G61 1QH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE78001 |
Expression changes in lymphoid cells on excision of Runx1 |
|
