|
| Status |
Public on Aug 31, 2016 |
| Title |
setdb1 mutant ovaries, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila ovaries at the same developmental stage.
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: ovary
genotype: setdb1 mutant
age: less than 5 days
|
| Treatment protocol |
Flies were fed with yeast and dissected the next day in PBS.
|
| Growth protocol |
Flies were incubated at 25C and collected within 5 days of hatching
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction for total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
biotin, standard Affymetrix 3' IVT express kit protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix FS450 protocol, AGCC
|
| Scan protocol |
standard Affymetrix GCS3007G scanner protocol
|
| Description |
egg1473/Df(2R)MP-II
|
| Data processing |
Affymetrix Expression Console and GeneSpring GX, PLIER
|
| |
|
| Submission date |
Feb 19, 2016 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
[email protected]
|
| Phone |
518-591-7215
|
| Organization name |
SUNY-University at Albany
|
| Department |
Center for Functional Genomics
|
| Lab |
Microarray Core
|
| Street address |
One Discovery Drive, CRC 342G
|
| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE78080 |
Expression data from bam and setdb1 mutant ovaries |
|
