|
| Status |
Public on Feb 26, 2016 |
| Title |
T_ALL_patient_39 [GPL97] |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
patient age at diagnosis: 18
all type: mature T-ALL
|
| Treatment protocol |
Pretreatment bone marrow mononuclear cells from patients with newly diagnosed acute T-cell lymphoblastic leukemia (T-ALL)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mononuclear cells from bone marrow (BM) were enriched by Ficoll gradient centrifugation. Total RNA was isolated using TRIzol (Life Technologies, Grand Island, NY, USA). cDNA preparation and in vitro transcription were performed according to standard Affymetrix protocols.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling of antisense cRNA was performed according to standard Affymetrix protocols
|
| |
|
| Hybridization protocol |
Sample hybridisation was performed for 16 hours according to standard Affymetrix protocols
|
| Scan protocol |
Samples were scanned using an Affymetrix GeneChip scanner
|
| Data processing |
Normalization was performed using the Robust Multichip Average (RMA) method. Quality control consisted of visual inspection of the array image for artifacts, assessment of RNA degradation plots, and inspection of rank-vs.-residual plots after normalization and probe set summarization.
|
| |
|
| Submission date |
Feb 19, 2016 |
| Last update date |
Feb 26, 2016 |
| Contact name |
Lars Fransecky |
| E-mail(s) |
[email protected]
|
| Phone |
+493084452338
|
| Organization name |
Charité University Hospital Berlin
|
| Department |
Hematology and Oncology
|
| Street address |
Hindenburgdamm 30
|
| City |
Berlin |
| ZIP/Postal code |
12203 |
| Country |
Germany |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE78132 |
Silencing of GATA3 defines a novel stem cell like subgroup of ETP-ALL |
|
