|
| Status |
Public on Feb 27, 2016 |
| Title |
10 min Pt(IV)chloride 50 µM, rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
cells of C. metallidurans
|
| Organism |
Cupriavidus metallidurans CH34 |
| Characteristics |
genotype/variation: wild type
|
| Growth protocol |
C. metallidurans cultures were grown in Mineral salts medium + 0.2 % Na-gluconate to a cell turbidity of 100 Klett, metal species added, incubated for 10-60 min, then harvest cells by centrifugation for 1' in 2.0 ml Tubes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) was used according to the manufacturers manual including on-column DNase digestion. PCR were done to check for DNA contaminations, only RNA was used that did not generate products in the PCR reaction. RNA quality was checked on formamide gels and measured as RIN on Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany).
|
| Label |
Cy3
|
| Label protocol |
Labeling was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| |
|
| Hybridization protocol |
Hybridisation was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Scan protocol |
Agilent DNA microarray Scanner were used by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Description |
RS-224_15
|
| Data processing |
The mean intensity of the pixels of the surrounding area was subtracted from the mean density of the pixels of the spots to give the signal strength
|
| |
|
| Submission date |
Feb 26, 2016 |
| Last update date |
Feb 27, 2016 |
| Contact name |
Cornelia Große |
| E-mail(s) |
[email protected]
|
| Organization name |
Martin-Luther-University Halle-Wittenberg
|
| Department |
Faculty of Biology/Microbiology
|
| Street address |
Kurt-Mothes-Str.3
|
| City |
Halle/Saale |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16115 |
| Series (1) |
| GSE78723 |
Response of Cupriavidus metallidurans CH34 to different Pt-species |
|
