|
| Status |
Public on Mar 25, 2019 |
| Title |
XY Control_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
E14.5 XY gonads
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed 129Sv and C57Bl/6J
tissue: Fetal gonads
age: E14.5
genotype: XY Rosa26+/+; Sf1-Cre+/Tg
|
| Treatment protocol |
All embryos used in this study resulted from the crossing between Rosa26-CAG-LSL-Foxl2+/f and Sf1-cre+/Tg mice. The genotype of the gonads used in this study is as follows: XX and XY control: Rosa26+/+; Sf1-Cre+/Tg and XY FOXL2+: XY Rosa26-CAG-LSL-Foxl2+/f; Sf1-cre+/Tg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A single pair of gonads (14.5 dpc) was separated from the mesonephros and was used as one biological replicate (4 biological replicates were included for each genotype). Total RNA was isolated using Picopure extraction kit (Arcturus). RNA quality and concentration were determined using N Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, California)
|
| Label |
biotin
|
| Label protocol |
One hundred nanograms (100 ng) of total RNA were amplified and labeled as directed using the Affymetrix WT Plus Reagent Kit (WT Plus Kit).
|
| |
|
| Hybridization protocol |
5.5 μg micrograms of amplified biotin-cDNAs were fragmented and hybridized to each array for 16 hours at 45°C in a rotating hybridization oven. Array slides were stained with streptavidin/phycoerythrin then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual protocol FS450-0001.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
Gene expression from E14.5 XY gonads
|
| Data processing |
Data was obtained using the GeneChip® Command Console software.
|
| |
|
| Submission date |
Mar 01, 2016 |
| Last update date |
Mar 25, 2019 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE78774 |
Reprogramming of somatic cell fate in fetal mouse testis by FOXL2 |
|
