|
| Status |
Public on Jul 07, 2007 |
| Title |
T02-136(2)-2 Control RNA - mAdbID:42661 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
T02-136(2)-2 Control RNA
|
| Organism |
Homo sapiens |
| Characteristics |
Strain: athymic nude mouse; Tissure: Tumor; Cell type: human breast cancer cell line Xenografted into nude mice; Disease state: breast cancer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction method: Trizol Other: Total RNA was extracted using Trizol according to manufacturer's instructions (Invitrogen, Carlsbad, CA).
|
| Label |
Cy 5
|
| Label protocol |
Sample Labeling Protocol - generic
|
| |
|
| Channel 2 |
| Source name |
Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
Equal quantities of total RNA from each cell line (brain, breast, B-lymphocyte, cervix, liver, liposarcoma, macrophages, skin, testis, Y-lymphocyte) were pooled together.
|
| Biomaterial provider |
Stratagene (La Jolla, CA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction method: Trizol Other: Total RNA was extracted using Trizol according to manufacturer's instructions (Invitrogen, Carlsbad, CA).
|
| Label |
cy 3
|
| Label protocol |
Sample Labeling Protocol - generic
|
| |
|
| |
| Hybridization protocol |
FairPlay Microarray Labeling Kit Other: Use the FairPlay Microarray Labelling Kit instruction from Stratagen, CA.
|
| Scan protocol |
GenePix 4000B
|
| Description |
mAdb experiment ID: 42661
|
| Data processing |
After background correction and removal of flagged values, log base 2 expression ratios were median centered and linear transformed to obtain the log and linear values given in the data table.
|
| |
|
| Submission date |
Jul 06, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
binwu tang |
| E-mail(s) |
[email protected]
|
| Phone |
3014968344
|
| Fax |
3014968395
|
| Organization name |
nih
|
| Department |
nci
|
| Lab |
lcrc
|
| Street address |
9000 rockville pike
|
| City |
bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1262 |
| Series (1) |
| GSE5265 |
TGF-beta on breast cancer cell |
|
