|
| Status |
Public on Aug 31, 2016 |
| Title |
HUVECs exposed to DMSO_2 |
| Sample type |
RNA |
| |
|
| Source name |
HUVECs exposed to DMSO
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human umblical vein endothelial cells (HUVECs)
exposed to: DMSO (control)
|
| Treatment protocol |
Cells were exposed to either DMSO (control vehicle) or the PCB mixture, Aroclor 1260
|
| Growth protocol |
Human umbilical vein endothelial cells (HUVECs, 10-donor pool) were obtained from Lifeline Cell Technology (Walkersville, MD, USA). Cells were maintained in VascuLife Basal Medium supplemented with components of the VascuLife VEGF LifeFactors Kit (containing Vascular Endothelial Growth Factor, 2% fetal bovine serum and other appropriate life factors) and 1% antimicrobial supplement (penicillin, streptomycin and amphotericin B). Cells were passaged and maintained according to the company instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA was labeled using Affymetrix Flashtag Biotin HSR RNA Labeling kit (P/N 901910, Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
450ng of biotin labeled RNA was hybridized to Affymetrix GeneChip® miRNA 4.0 Arrays at 48 °C and 60 rpm for 16-18 h.
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
MicroRNA profile data
|
| Data processing |
affymetrix-algorithm-name = rma : dabg
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Mar 08, 2016 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Banrida Wahlang |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Kentucky
|
| Department |
UK Superfund Research Center
|
| Street address |
900 S Limestone Street Rm 559
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE79005 |
Profile of microRNAs in human umblical vein endothelial cells (HUVECs) |
|
