|
| Status |
Public on Dec 31, 2016 |
| Title |
syncytiotrophoblast (ST) Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
term placenta 1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: in vitro-differentiated syncytiotrophoblast
culture time: 72h
|
| Growth protocol |
VCT were isolated from term placenta by trypsin and purified on percoll gradient, then plated onto Petri dishes in DMEM/10% FBS, cultured for 12h (VCT) or 72h (ST). VCT/ST pellets were then snap-frozen and stored at -80°C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy mini kit (Qiagen) following manufacturer's instructions
amplification protocol: One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion)
|
| Label |
Cy5
|
| Label protocol |
Three micrograms of amplified RNA (aRNA) was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences)
|
| |
|
| Channel 2 |
| Source name |
term placenta 1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: total placental extract
|
| Growth protocol |
VCT were isolated from term placenta by trypsin and purified on percoll gradient, then plated onto Petri dishes in DMEM/10% FBS, cultured for 12h (VCT) or 72h (ST). VCT/ST pellets were then snap-frozen and stored at -80°C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy mini kit (Qiagen) following manufacturer's instructions
amplification protocol: One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion)
|
| Label |
Cy3
|
| Label protocol |
Three micrograms of amplified RNA (aRNA) was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences)
|
| |
|
| |
| Hybridization protocol |
The labeled cDNA mixtures were hybridized according to the protocol described at http://cmgm.stanford.edu/pbrown/protocols/index
|
| Scan protocol |
The arrays were immediately scanned using a GenePix 4000A confocal Scanner (Axon Instruments). Images were analyzed using GenePix pro 3 software
|
| Description |
Biological replicate 1 of 5. Syncytiotrophoblast vs placenta
amplfied RNA
|
| Data processing |
A print-tip loess normalization of the microarray experiments was performed after the log-transformation of the background-corrected expression measurements
|
| |
|
| Submission date |
Mar 17, 2016 |
| Last update date |
Dec 31, 2016 |
| Contact name |
Severine Aude Degrelle |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM UMRS-1139
|
| Street address |
4 avenue de l'Observatoire
|
| City |
PARIS |
| ZIP/Postal code |
75006 |
| Country |
France |
| |
|
| Platform ID |
GPL21609 |
| Series (1) |
| GSE79333 |
Transcriptomic signatures of villous cytotrophoblast and syncytiotrophoblast in term human placenta. |
|
