|
| Status |
Public on Jun 15, 2016 |
| Title |
bind_RocA3_ADP_replicate2 |
| Sample type |
SRA |
| |
|
| Source name |
randomized synthetic RNAs
|
| Organism |
synthetic construct |
| Characteristics |
treatment: RocA 3 uM
cell line: None
extracted molecule: randomized synthetic RNAs
extract_protocol: randomized synthetic RNAs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction, preadenylated linker ligation, reverse transcription, cDNA circularization, PCR amplification
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Bind-n-Seq
|
| Data processing |
Basecalling with Illumina Casava 1.8 software
3' adapter trimming with FastX-toolkit
Depleting duplicated reads with Fast-X-toolkit
fasta files, reads named with "id number" - "duplication number of the read"
|
| |
|
| Submission date |
Mar 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Shintaro Iwasaki |
| E-mail(s) |
[email protected]
|
| Organization name |
RIKEN
|
| Department |
Cluster for Pioneering Research
|
| Street address |
S205 Bioscience Bldg. 2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21616 |
| Series (1) |
| GSE79392 |
Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor |
|
| Relations |
| BioSample |
SAMN04568459 |
| SRA |
SRX1642579 |
