|
| Status |
Public on Jul 13, 2007 |
| Title |
Hippocampus_Rasgrf1KO_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus from mouse Rasgrf1 knockout
|
| Organism |
Mus musculus |
| Characteristics |
Laser Capture Microdissection (LCM) to isolate tissue sections of the hippocampus area of Rasgrf1 knockout mice brains
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNAs extracted from microdissected tissue sections were pre-amplified prior to microarray hybridization using the Gene Chip Expression 3′-Amplification Two-Cycle cDNA Synthesis kit (Affymetrix, Santa Clara, CA, USA; #900432), the Gene Chip Sample Cleanup Module (Affymetrix #900371) and the MEGAscript T7 High Yield Transcription Kit (Ambion, Austin, TX, USA; #1334), according to Affymetrix instruction manual #701025 rev. 5.
|
| Label |
Standard Gene Chip microarray hybridization protocol
|
| Label protocol |
Gene Chip microarray label protocol (Affymetrix Expression Analysis Technical Manual, http://www.affymetrix.com/support/technical/manual/expression_manual.affx).
|
| |
|
| Hybridization protocol |
Gene Chip microarray hybridization protocol (Affymetrix Expression Analysis Technical Manual).
|
| Scan protocol |
Gene Chip microarray scan protocol (Affymetrix Expression Analysis Technical Manual).
|
| Description |
Laser Capture Microdissection (LCM) to isolate tissue sections of the hippocampus area of Rasgrf1 knockout mice brains
|
| Data processing |
GCOS
|
| |
|
| Submission date |
Jul 10, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Javier De Las Rivas |
| E-mail(s) |
[email protected]
|
| Phone |
34 923 294819
|
| Organization name |
Cancer Research Center (CiC-IBMCC)
|
| Department |
CSIC and University of Salamanca
|
| Lab |
Bioinformatics and Functional Genomics
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| ZIP/Postal code |
37001 |
| Country |
Spain |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE8425 |
Laser microdissection and microarray analysis of the hippocampus of Rasgrf1 knockout mice |
|
