|
| Status |
Public on Mar 29, 2016 |
| Title |
white 1118 embryos at 10-13 stage, control, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
dechorionated embryo
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: dechorionated embryo
genotype: white 1118 embryos
developmental stage: 10-13 stage, from germ band retraction to finish dorsal closure process
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from aproximated 1000 dechorionated cbtEP(2)2237E1 and Cbt>69B embryos at 10-13 stage and purified with the mrVANATM miRNA insolation kit (Ambion·AM1260) following manufacturer´s instructions. RNA quality and quantification was performed in a Biorad Experion bioanalyzer. Three cbtEP(2)2237E1, three w1118 and three 69B>Cbt RNA samples were prepared and used to hybridize to Drosophila Genome 2.0 Affymetrix Microarrays following manucaturer´s instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was label with Biotin according to standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization was performed following Affymetrix protocol, chips were washed and stained using Fluidics protocol midiEuk2v3_450 on an Affymetrix GeneChip Fluidics Station 450
|
| Scan protocol |
Affymetrix scanner GeneChip 3000 7G was used to read the arrays
|
| Description |
Expression profiling by array
|
| Data processing |
Pre-processing of data was performed using the RMA (robust Multi-Array) function of the affy package and differential expression analysis using LIMMA (Lineal Models for Microarray Data) package, both from Bioconductor (
|
| |
|
| Submission date |
Mar 28, 2016 |
| Last update date |
Mar 29, 2016 |
| Contact name |
YAIZA BELACORTU |
| E-mail(s) |
[email protected]
|
| Phone |
+34 652375801
|
| Organization name |
UNIVERSITY OF VALENCIA
|
| Department |
GENETICS
|
| Lab |
MOLECULAR DEVELOPMENTAL GENETICS
|
| Street address |
DR. MOLINER
|
| City |
BURJASSOT |
| State/province |
VALENCIA |
| ZIP/Postal code |
46100 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE79646 |
Expression data from Drosophila mutants´ (misexpression and overexpression) of cabut/dTIEG gene |
|
