|
| Status |
Public on Jul 14, 2007 |
| Title |
110spc4 micro PSY 13 |
| Sample type |
RNA |
| |
|
| Source name |
B. japonicum wild-type strain grown microaerobically
|
| Organism |
Bradyrhizobium japonicum |
| Characteristics |
Genotype: 110spc4
Growth condition: micro
Medium: PSY
|
| Treatment protocol |
no treatment was applied
|
| Growth protocol |
cells were grown until OD600 0.4-0.5 in PSY medium or as soybean bacteroids
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cells were prepared as described by Pessi et al.(2007) accepted manuscript
|
| Label |
biotin
|
| Label protocol |
2.8 mg (free-living cultures) and 7.8 mg (nodules) cDNA were labeled with biotin
|
| |
|
| Hybridization protocol |
Modification of Affymetrix protocol used for P. aeruginosa (2-2.5 mg of free-living cultures and 6-7.5 mg of nodules cDNA were hybridised)
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Gene expression data from B. japonicum wild-type strain grown microaerobically
|
| Data processing |
GeneChip Operating Software (GCOS) version 1.1 (Affymetrix), global scaling to a target intensity of 500
|
| |
|
| Submission date |
Jul 13, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Hubert Rehrauer |
| E-mail(s) |
[email protected]
|
| Organization name |
ETH Zurich / University of Zurich
|
| Department |
FGCZ
|
| Street address |
Winterthurerstr. 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL3401 |
| Series (4)
|
| GSE8478 |
Genome-wide transcript analysis of Bradyrhizobium japonicum bacteroids in soybean root nodules |
| GSE9100 |
Comparison analysis of B. japonicum mutant and wild type grown under different conditions |
| GSE12491 |
Comprehensive assessment of the regulons controlled by the FixLJ-FixK2-FixK1 cascade in Bradyrhizobium japonicum |
| GSE21400 |
Autoregulation of fixK2 gene expression in Bradyrhizobium japonicum |
|
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