|
| Status |
Public on May 21, 2017 |
| Title |
blood_cDC1_3 |
| Sample type |
RNA |
| |
|
| Source name |
blood_cDC1
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: donor 3
subject status: healthy
cell type: Peripheral Blood Mononuclear Cells (PBMCs)
cell subtype: cDC1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from FACS-sorted blood pre-DC and DC subsets with the Qiagen Rneasy Micro Kit (Qiagen) according to the manufacturer's protocol. Total RNA integrity was assessed using Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the protocol by Epicentre TargetAmpTM 2-Round Biotin-aRNA Amplification Kit 3.0 using 500pg of total RNA.
|
| |
|
| Hybridization protocol |
Hybridization of cRNA was performed on Illumina Human-HT12 Version 4 chips.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
cDC1 isolated from PBMC of healthy donor 3
|
| Data processing |
Probe level expression data were exported from Illlumina GenomeStudio software without background subtraction. These raw data were log (base 2) transformed and quantile normalized in R using Bioconductor lumi package.
|
| |
|
| Submission date |
Apr 12, 2016 |
| Last update date |
May 21, 2017 |
| Contact name |
Kaibo Duan |
| E-mail(s) |
[email protected]
|
| Organization name |
Singapore Immunology Network
|
| Street address |
8A Biomedical Grove, Immunos Building, Level 4
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE80171 |
Transcriptome profiles of human pre-DC and DC subsets in peripheral blood |
|
