|
| Status |
Public on Jul 16, 2008 |
| Title |
SarZ mutant biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
SarZ mutant biological rep 2
|
| Organism |
Staphylococcus epidermidis |
| Characteristics |
SarZ mutant
|
| Growth protocol |
For microarray experiments, overnight cultures were diluted 1:100 into 50 ml of TSB and incubated at 37℃ with shaking at 180rpm until grown to the late exponential phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by use of an RNeasy Mini Kit (Qiagen) recommended by a standard protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 40C on our custom Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus.
|
| Description |
Gene expression data from_SarZ mutant biological rep 2
|
| Data processing |
The data were analyzed with GCOS 1.4 using Affymetrix default analysis with a scale filter for Se genes setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jul 19, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
[email protected]
|
| Phone |
4063639248
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
RTS
|
| Street address |
903 S 4th street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL4692 |
| Series (1) |
| GSE8523 |
SarZ is a key regulator of biofilm formation and virulence in Staphylococcus epidermidis |
|
