|
| Status |
Public on Oct 11, 2016 |
| Title |
MMTV_PyMT_375_Macro |
| Sample type |
RNA |
| |
|
| Source name |
tumor
|
| Organism |
Mus musculus |
| Characteristics |
tumor type: Breast cancer
model type: Genetically Engineered Model
cell type: Mouse macrophages (M1+M2)
mouse type: FVB
patient id: N/A
|
| Treatment protocol |
Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
|
| Growth protocol |
Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
|
| Scan protocol |
GeneChips were scanned in the Affymetrix GeneTitan.
|
| Description |
Breast cancer GEM, Tumor 375
|
| Data processing |
Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
|
| |
|
| Submission date |
Apr 18, 2016 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
[email protected]
|
| Organization name |
GenoSplice technology
|
| Department |
Paris Biotech Santé
|
| Street address |
29, rue du Faubourg Saint-Jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE80410 |
Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts |
|
