|
| Status |
Public on Oct 23, 2007 |
| Title |
classIIhiMECs B6aireWT rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MHC class II high thymic medullary epithelial cells, B6aireWT
|
| Organism |
Mus musculus |
| Characteristics |
Gender: male
Age: 3-4.5 weeks
Isolation: FACS of CD45-G8.8+Ly51intclassIIhi TECs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
MHC class II high thymic medullary epithelial cells, B6aireWT
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jul 23, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
[email protected]
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE8563 |
Lymphtoxin pathway effects on thymic medullary epithelial cells |
| GSE8564 |
Analysis of Aire effects on individual mice of different genetic backgrounds |
|
| Relations |
| Reanalyzed by |
GSE119085 |
