|
| Status |
Public on Aug 02, 2008 |
| Title |
HUVEC cells co-cultured with 1205Lu melanoma cells |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC cells co-cultured with 1205Lu melanoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
RFP-labeled HUVEC primary cells
|
| Biomaterial provider |
Cambrex
|
| Treatment protocol |
RFP-labeled HUVEC cells were co-cultured with 1205Lu melanoma cells for 48 hours in EGM-2 medium.
|
| Growth protocol |
Cells were grown in EGM-2 medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit (QIAGEN)
|
| Label |
Biotin
|
| Label protocol |
One round amplification protocol for total RNA following Affymetrix’ specifications using T7 promoter oligo dT primer for cDNA synthesis, and ENZO IVT kit (www.affymetrix.com, GeneChip Expression Analysis, Technical Manual, 2003).
|
| |
|
| Hybridization protocol |
16hrs at 45 C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual, 2004.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual, 2004.
|
| Description |
Image analysis using GCOS 1.4, using MAS 5.0 algorithm and manufacturer’s specifications. Global scaling of images to a target intensity of 150.
|
| Data processing |
Affymetrix MAS5
|
| |
|
| Submission date |
Aug 03, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Megan Jean Stine |
| E-mail(s) |
[email protected]
|
| Phone |
410 614 9309
|
| Organization name |
The Johns Hopkins University School of Medicine
|
| Street address |
1650 Orleans Street, CRB1 336
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8699 |
Characterization of the gene expression changes associated with melanoma-endothelial cell communication |
|
| Relations |
| Reanalyzed by |
GSE119087 |
