|
| Status |
Public on Aug 01, 2008 |
| Title |
crol_females_1 |
| Sample type |
RNA |
| |
|
| Source name |
whole bodies of 42 day old crol female flies, replicate 1
|
| Organism |
Drosophila melanogaster |
| Characteristics |
whole bodies of 42 day old crol female flies, replicate 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's (Affymetrix) instructions
|
| Label |
Biotinylated cRNA
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 mkg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Seven lines homozygous for a single P-element insertion led to an increase in life span. Two replicates of each sex and line were used to identify differences in expression from the isogenic control line. Flies were aged 6 weeks (42 days) and frozen at approximately the same time (10:30 -12:00 AM) for RNA extraction.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Aug 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mike Magwire |
| E-mail(s) |
[email protected]
|
| Phone |
919-515-5770
|
| Organization name |
NCSU
|
| Department |
Genetics
|
| Lab |
Mackay
|
| Street address |
3501 Gardner Hall
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE8775 |
Mutations Increasing Drosophila melanogaster Life Span |
|
| Relations |
| Reanalyzed by |
GSE119084 |
