|
| Status |
Public on Aug 09, 2008 |
| Title |
KINK-1 24 hrs., 1 |
| Sample type |
RNA |
| |
|
| Source name |
melanoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
A375 cell line
|
| Treatment protocol |
A375 melanoma cells were grown in DMEM-medium to subconfluency, then treated for 12hrs / 24hrs with 5ul KINK-1
|
| Growth protocol |
A375 melanoma cells were grown in DMEM-medium to subconfluency
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated using the Rneasy Mini Kit from Quiagen (including Dnase digest)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (GeneChip® Expression Analysis Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on HG U 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000
|
| Description |
Gene expression data from melanoma cells A375, treated with 5 u molar KINK-1 for 24 hrs
|
| Data processing |
The data were analyzed with Bioconductor packages (under R) and vsn as normalization method using perfect match only.
|
| |
|
| Submission date |
Aug 14, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Margarete Schoen |
| E-mail(s) |
[email protected]
|
| Organization name |
RVZ
|
| Street address |
Versbacherstr.9
|
| City |
Wuerzburg |
| ZIP/Postal code |
97078 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8772 |
KINK-1, a novel small-molecule inhibitor of IKKbeta, enhances susceptibility of melanoma cells to antitumoral treatment |
|
| Relations |
| Reanalyzed by |
GSE119087 |
