|
| Status |
Public on Feb 01, 2017 |
| Title |
E11.5_placenta_miRNA_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic day 11.5, placenta, IVF derived, microRNA, replicate3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: placenta
embryonic day: 11.5
|
| Growth protocol |
In vitro-fertilized embryos derived from wild-type and miRNA-deleted mice were cultured in KSOM medium at 37°C for 16 hours. Cultured embryos were transferred into pseudopregnant ICR females and E11.5 embryos were retrieved from the uterus.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Placentas with deciduas removed were lysed and the total and miRNA isolated using a mirVana miRNA isolation kit (Themo Fisher Scientific).
|
| Label |
Cy3
|
| Label protocol |
For miRNA analysis, total RNA from placentas was labeled with the miRNA Complete Labeling Reagent and Hyb Kit (Agilent Technologies). For mRNA analysis, total RNAs were labeled with a Low Input Quick Amp Labeling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
For miRNA analysis, Cy3-labeled RNAs was hybridized with the miRNA Complete Labeling Reagent and Hyb Kit onto mouse miRNA Microarray rel.17.0 (Agilent Technologies) at 65°C for 17 hours according to manufacture's instructions. For mRNA analysis, 0.6ug Cy3-labelled RNAs were fragmented at 60°C for 30 minutes and hybridized onto SurePrint G3 Mouse GE microarray 8x60K (Agilent Technologies) at 65°C for 17 hours according to manufacture's instructions.
|
| Scan protocol |
Scanning of microarray slides was performed with a DNA microarray scanner at 3 um resolution (Agilent Technologies).
|
| Description |
embryonic day E11.5, mouse, placenta, in vitro fertilized embryo, microRNA, replicate3
|
| Data processing |
Scanned image files were processed to give signal intensities using Feature Extraction software (Agilent Technologies). miRNA raw intensity signals were not normalized.
|
| |
|
| Submission date |
May 31, 2016 |
| Last update date |
Feb 01, 2017 |
| Contact name |
Kimiko Inoue |
| E-mail(s) |
[email protected]
|
| Organization name |
BRC, RIKEN
|
| Department |
Bioresource Engineering Division
|
| Street address |
3-1-1 Koyadai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-0074 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15547 |
| Series (2) |
| GSE82054 |
The Rodent-Specific MicroRNA Cluster within the Sfmbt2 Gene is Imprinted and Essential for Placental Development [miRNA] |
| GSE82055 |
The Rodent-Specific MicroRNA Cluster within the Sfmbt2 Gene Is Imprinted and Essential for Placental Development |
|
