|
| Status |
Public on Jun 14, 2017 |
| Title |
NIPPONBARE_drought_stressed_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
NIPPONBARE_drought_stressed
|
| Organism |
Oryza sativa |
| Characteristics |
variety: NIPPONBARE
developmental stage: reproductive development
treatment group: drought-stressed
tissue: young panicles (2 cm in length)
|
| Treatment protocol |
Drought treatments were conducted at later tillering stage and allowed to dry.
|
| Growth protocol |
Rice grew in cement enclosed plots.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The young panicles in 2 cm length were harvested for RNA extraction.
|
| Label |
biotin
|
| Label protocol |
Five mg of total RNA was reverse-transcribed to cDNA, and then 2 μg cDNA was used for probe labeling according to the instructions of Affymetrix Rice Genome Array.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
610
|
| Data processing |
The raw data were processed with RMA function in Affy package of the BioConductor and differential gene expression was assessed using limma package on the Bioconductor.
|
| |
|
| Submission date |
Jun 15, 2016 |
| Last update date |
Jun 14, 2017 |
| Contact name |
haibin wei |
| E-mail(s) |
[email protected]
|
| Phone |
86-21-54920904
|
| Organization name |
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
|
| Department |
Institute for Nutritional Sciences
|
| Lab |
Key Laboratory of Nutrition and Metabolism
|
| Street address |
294 Taiyuan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE83378 |
The drought-responsive gene expression in rice panicle |
|
