|
| Status |
Public on Jun 13, 2018 |
| Title |
CD30 B GC_G |
| Sample type |
RNA |
| |
|
| Source name |
CD20+CD30+CD38+ B cells isolated by cell sorting from a tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tonsil
cell type: B cells
cell subtype: CD20+CD30+CD38+ B cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the Purescript RNA isolation kit from Gentra, according to the protocol of the supplier, reducing all volumes to one tenth of the volume recommended for a standard isolation
|
| Label |
biotin
|
| Label protocol |
Samples were labelled, washed and stained using the Labeling and HWS Kit from Affymetrix.
|
| |
|
| Hybridization protocol |
Fragmentation of cRNA, microarray hybridisation to Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays, washing steps of the microarrays was performed according to the Affymetrix protocol.
|
| Scan protocol |
Scanning was performed according to the Affymetrix protocol with Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
CD30_B_GC_G
|
| Data processing |
Statistical analysis was done with the statistical computing environment R. Additional software packages (affy, geneplotter, multtest, and vsn) were taken from the Bioconductor project. Microarray preprocessing: Probe level normalization (together with all samples from GSE records 12453, 14879 and 40160) was conducted using the variance stabilization method (vsn) from Huber et al..
probe group file: Unsupported Human Genome U133 Plus 2.0 Array CDF Files
meta-probeset file: Human Genome U133 Plus 2.0 Probeset Annotations, CSV Format, Release 33
|
| |
|
| Submission date |
Jun 16, 2016 |
| Last update date |
Jun 13, 2018 |
| Contact name |
Claudia Döring |
| E-mail(s) |
[email protected]
|
| Organization name |
Johann Wolfgang-Goethe University Frankfurt a.M.
|
| Department |
Medical School, Institite of Pathology
|
| Street address |
Theodor Stern Kai 7
|
| City |
Frankfurt |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE83441 |
Characterization of human CD30+ B cells and their relationship to Hodgkin and Reed-Sternberg cells of Hodgkin lymphoma |
|
