|
| Status |
Public on Dec 05, 2016 |
| Title |
Calment1/4, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Calment1/4_whole adult females
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: Calment1/4 mutant (in CS10 background)
developmental stage: adult
gender: female
|
| Treatment protocol |
Adult Calment1/4 and CanS females were collected (30-35 flies per sample) and flash frozen in liquid Nitrogen
|
| Growth protocol |
Calment1/4 and CanS flies were cultured in standard Drosophila medium in a 25°C incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted in TRI-Reagent (Sigma) according to manufacturer's instructions, followed by a second round of RNA precipitation with 10 M ammonium acetate and ethanol before resuspending RNA pellet in water.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from total RNA according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 µg of cRNA was hybridized for 16 hr at 45°C on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
| Description |
Calment 3
|
| Data processing |
Data was analyzed with the affy package (version 1.36.0) of BioConductor (www.bioconductor.org) to obtain RMA expression values. MAS 5.0 detection calls (Affymetrix) were used to determine if signals were significantly above background.
|
| |
|
| Submission date |
Jun 22, 2016 |
| Last update date |
Dec 05, 2016 |
| Contact name |
Patrick Callaerts |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Leuven
|
| Department |
Department of Human Genetics
|
| Lab |
Laboratory of Behavioral and Developmental Genetics
|
| Street address |
Herestraat 49, bus 602
|
| City |
Leuven |
| ZIP/Postal code |
B-3000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE83605 |
The role of Calment in Drosophila longevity and oxidative stress resistance |
|
