|
| Status |
Public on Jun 21, 2017 |
| Title |
BEAS-2B TTF-1, treated with doxycycline for 48h |
| Sample type |
RNA |
| |
|
| Source name |
BEAS-2B cell line stably transduced with a DOX-inducible TTF-1 expression vector, treated with doxycycline for 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BEAS-2B
protocol: transduced with a DOX-inducible TTF-1 expression construct
treatment: doxycycline
|
| Treatment protocol |
BEAS-2B CTRL and TTF-1 cell lines were treated with 1 ug/ml Doxycycline for 48 h before RNA was harvested
|
| Growth protocol |
BEAS-2B cells were cultured with 1% (v/v) FCS-containing Ham F-12 supplemented with bovine insulin (5 ug/mL), human transferrin (5 ug/mL), 100 nM hydrocortisone, 200 pM triiode thyronine, penicillin (100 IU/mL), and streptomycin (100 ug/mL) at 37°C with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNeasy kit (Qiagen, Valencia, CA)
|
| Label |
FAM
|
| Label protocol |
Total RNA was converted into cDNA using the megaplex reverse transcriptase reaction. The megaplex reaction contained a specific stem-loop primer for each of the mature target microRNA's. The cDNA was subjected to a pre-amplification PCR. The diluted pre-amplification products served as substrates for TaqMan quantitative PCR reactions.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
BEAS-2B TTF-1 48h miRNA
|
| Data processing |
Normalization was carried out with the expression level of RNU44.
|
| |
|
| Submission date |
Jun 29, 2016 |
| Last update date |
Jun 21, 2017 |
| Contact name |
Takashi Takahashi |
| Organization name |
Aichi Cancer Center
|
| Street address |
1-1 Kanokoden, Chikusa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8681 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11316 |
| Series (2) |
| GSE83838 |
TTF-1-regulated miR-532-5p targets KRAS and MKL2 oncogenes and induces apoptosis in lung adenocarcinoma [rt-pcr] |
| GSE83839 |
TTF-1-regulated miR-532-5p targets KRAS and MKL2 oncogenes and induces apoptosis in lung adenocarcinoma |
|
