|
| Status |
Public on Jul 18, 2016 |
| Title |
O3 |
| Sample type |
RNA |
| |
|
| Source name |
aged replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
cell type: rat primordial follicles
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from immature and aged rat primordial follicles was measured by Qubit® Fluorometer. The quality of RNA was analyzed by using Agilent RNA 6000 Nano Kit and Agilent 2100 electrophoresis Bio-anlyzer according to the manufacturer’s instructions by subjecting 1µl of the diluted RNA on to a Nano chip. The quality of total RNA was obtained as electropherogram profile and RNA integrity number (RIN) generated by the Bioanalyzer software.
|
| Label |
Cy3
|
| Label protocol |
biotinylated cRNA using Illumina TotalPrep RNA Amplification kit (Ambion, UK)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 13, 2016 |
| Last update date |
Jul 18, 2016 |
| Contact name |
madavan vasudevan |
| E-mail(s) |
[email protected]
|
| Phone |
+91-9845093355
|
| Organization name |
Theomics International Private Limited
|
| Street address |
Kasturi Nagar
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560043 |
| Country |
India |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE84333 |
Age-related changes in gene expression patterns of immature and aged rat primordial follicles |
|
