|
| Status |
Public on Oct 07, 2016 |
| Title |
H2A T120D #6 |
| Sample type |
SRA |
| |
|
| Source name |
fibrobrast
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic fibrobrast cells
cell line: NIH/3T3
expression: EGFP-H2A T120D
|
| Growth protocol |
NIH/3T3 cells in which human H2A wild type or T120D mutant was integrated were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10 µg/ml gentamicin and 10% fetal calf serum (Thermo Fisher Scinetific).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using ISOGEN II reagent (NIPPON GENE), according to the manufacturer’s protocol.
RNA-seq libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Basecalls performed using Illumina MiSeq
RNA-seq reads were aligned to the Mouse mm9 genome assembly using Illumina MiSeq
RPKM were calculated using Partek Genomic suite version 6.6
Genome_build: Mouse mm9
|
| |
|
| Submission date |
Jul 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
TAKEYA NAKAGAWA |
| E-mail(s) |
[email protected]
|
| Organization name |
Nagasaki unversity
|
| Department |
Biochemistry
|
| Street address |
1-12-4 sakamoto
|
| City |
Nagasaki |
| ZIP/Postal code |
852-8523 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16417 |
| Series (2) |
| GSE84527 |
Histone H2A T120 phophorylation promotes oncogenic transformation via upregulation of cyclin D1 [RNA-seq] |
| GSE84968 |
Histone H2A T120 phophorylation promotes oncogenic transformation via upregulation of cyclin D1y |
|
| Relations |
| BioSample |
SAMN05414151 |
| SRA |
SRX1959103 |
