|
| Status |
Public on Feb 08, 2017 |
| Title |
Control r5 |
| Sample type |
RNA |
| |
|
| Source name |
Young control mice that were single-housed in a cage without a running wheel. These young mice have normal hearing
|
| Organism |
Mus musculus |
| Characteristics |
strain: CBA/CaJ
tissue: cochlea
developmental age: 6 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Inner ear tissues were frozen in liquid nitrogen and then ground into a powder in a 1.5 ml tube using a tissue homogenizer (Kimble-Chase, Vineland, NJ). Total RNA from the powdered tissue was isolated using the NucleoSpin RNA II kit
|
| Label |
biotin
|
| Label protocol |
100 ng total RNA was labeled using GeneChip® WT PLUS Reagent Kit (WT PLUS Kit) from Affymetrix following manufacturer's instructions
|
| |
|
| Hybridization protocol |
5.5 ug labeled and fragmented cDNA were hybridized onto GeneChip®MTA_1.0
|
| Scan protocol |
Chips were washed in a FS 450 fluidics station and then scanned on GeneChip® Scanner 3000 7G following Affymetrix recommendations
|
| Data processing |
Data was normalized using RMA as implemented in Partek and later analyzed using BRB-Array tools
|
| |
|
| Submission date |
Jul 21, 2016 |
| Last update date |
Feb 08, 2017 |
| Contact name |
M Cecilia Lopez |
| E-mail(s) |
[email protected]
|
| Phone |
352 273-8133
|
| Organization name |
University of Florida
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Microarray lab 103
|
| Street address |
1376 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-3610 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE84682 |
Effects of Long-term Exercise on Age-related Hearing Loss in Mice |
|
