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Sample GSM225042 Query DataSets for GSM225042
Status Public on Nov 01, 2007
Title H1 embryonic stem cells - GFP
Sample type RNA
 
Source name H1 undifferentiated embryonic stem cells - GFP
Organism Homo sapiens
Characteristics derived from H1-GFP labeled
Treatment protocol see supplementary information Lu et.al, Nature Methods, 2007 Jun;4(6):501-9.
Growth protocol see supplementary information Lu et.al, Nature Methods, 2007 Jun;4(6):501-9.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by Qiagen RNAeasy kiit and was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Following fragmentation, cRNA was hybridized for 16 hr.
Scan protocol Once the probe array has been hybridized, washed, and stained, it is scanned.
Description H1-ES
Data processing The data were analyzed with Robust Multi-Chip Analysis (RMA).
 
Submission date Aug 27, 2007
Last update date Aug 28, 2018
Contact name Jennifer A Hipp
E-mail(s) [email protected]
Organization name Wake Forest School of Medicine
Department Institute for Regenerative Medicine
Lab Atala
Street address Medical Center Blvd
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
 
Platform ID GPL570
Series (2)
GSE8884 Expression data from human embryonic stem cells, early stage embryoid bodies, and hES-derived blast cells.
GSE9196 Comparison of ES, EB, and Blast cells to breast epithelial, leuckocytes, endothelial and stromal cells
Relations
Reanalyzed by GSM230247
Reanalyzed by GSM230268
Reanalyzed by GSM230280
Reanalyzed by GSM230291
Reanalyzed by GSM871460
Reanalyzed by GSE49910
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE normalized signal intensities of each chip by RMA

Data table
ID_REF VALUE
1007_s_at 8.34031347673648
1053_at 7.20354700964117
117_at 4.80131823855251
121_at 7.1297525562488
1255_g_at 6.5537788529173
1294_at 4.58806313447282
1316_at 5.25414392632116
1320_at 4.28353742028718
1405_i_at 2.61387040858256
1431_at 2.78436665317129
1438_at 5.23231598440374
1487_at 6.63264271230746
1494_f_at 4.85288197328025
1552256_a_at 7.59699104967189
1552257_a_at 8.50335167588082
1552258_at 3.95757017469969
1552261_at 4.46449997825479
1552263_at 4.77432351556658
1552264_a_at 6.81813318815702
1552266_at 3.28663330525364

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.




Supplementary file Size Download File type/resource
GSM225042_H1.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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