|
| Status |
Public on Aug 12, 2016 |
| Title |
B6*F*CD4_53 |
| Sample type |
RNA |
| |
|
| Source name |
CD4 T cells; female B6
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD4 T cells
Sex: female
strain: B6
|
| Treatment protocol |
none
|
| Growth protocol |
mice were maintained on standard chow and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNEasy kit was used to extract total RNA from FACS-sorted cells; RNA was converted to cRNA using the Illumina TotalPrep-96 RNA Amplification kit
|
| Label |
biotin
|
| Label protocol |
none
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using Illumina BeadArray Reader software
|
| Description |
B6*F*CD4
|
| Data processing |
Signal values from unambiguous probes were local background corrected and data across arrays were quantile normalized using the lumi package from Bioconductor
|
| |
|
| Submission date |
Aug 10, 2016 |
| Last update date |
Aug 12, 2016 |
| Contact name |
Dimitry Krementsov |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Vermont
|
| Department |
BHSC
|
| Street address |
312C Rowell bldg, 106 Carrigan drive
|
| City |
89 Beaumont ave |
| State/province |
VT |
| ZIP/Postal code |
05405 |
| Country |
USA |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE85418 |
Natural genetic variation profoundly regulates gene expression in immune cells and dictates susceptibility to CNS autoimmunity |
|
