|
| Status |
Public on Nov 10, 2007 |
| Title |
24 hrs infection 1 |
| Sample type |
RNA |
| |
|
| Source name |
airway epithelial cells A549 cells infected with hMPV for 24 h
|
| Organism |
Homo sapiens |
| Characteristics |
none
|
| Treatment protocol |
Cell monolayers were mock infected or infected with hMPV at MOI of 1 in serum-free medium containing 1 µg of trypsin/ml.RNAqueous®-Midi Kit (Ambion, Austin, TX),converted cDNA utilizing a T7-oligo (dT) primer was used as a template to synthesis biotinylated cRNA, using bacteriophage T7 RNA polymerase
|
| Growth protocol |
F12K medium containing 10% (v/v) FBS, 100 IU/ml penicillin and 100 μg/ml streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared accordiing to the standard Affymetrix protocol from 8.4 ug of total RNA
|
| |
|
| Hybridization protocol |
Follow fragmentation, 10ug of cRNA were hybridized for 16 hrs at 45C onto the HG U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
Gene Array Scanner (Hewlett Packard)
|
| Description |
Gene Expression data A549 cells hmpv infected for 24 h
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 05, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Antonella Casola |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
(409) 747-0581
|
| Organization name |
University of Texas Medical Branch(UTMB)
|
| Department |
Pediatrics
|
| Street address |
Children's Hospital. Rm#2.364, 301 University Blvd
|
| City |
Galveston |
| State/province |
TX |
| ZIP/Postal code |
77555-0366 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8961 |
Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
