|
| Status |
Public on Dec 31, 2007 |
| Title |
Y/W_Female_Drosophila melanogaster_Acetone_topical_Replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
Y/W_Female_Drosophila melanogaster_Acetone_topical_Replicate2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Strain: y,w Sex: females Stage: Adult Other: Flies were grown on regular yeast diet, switched to no-yeast food within one hour of eclosion, and yeast-starved for 5 days posteclosion
|
| Biomaterial provider |
Tatar Lab, Brown University, Providence
|
| Treatment protocol |
We anesthetized flies on ice and topically treated them with 0.1 microliter of 187 mM JH III in acetone or with 0.1? microliter 100% acetone (control) using a 1 ul Hamilton syringe with a repeating dispenser. 12 hours after hormone administration, samples were snap-frozen in liquid nitrogen and stored at -80°C. RNA was isolated from samples (2 JH samples, 2 control samples, each with 30 females) by lysis, as described in Gershman et al. (2007). cDNA products were hybridized at the Brown University Genomics Core Facility to Affymetrix GeneChip Drosophila_1 Genome Arrays (2 replicate chips per treatment). The dataset consisted of 14,009 probe sets, with 6,142 probe sets annotated. Expression data were analyzed for significant over- or underrepresentation of gene ontology (GO) terms with the web application FatiGO (Al-Shahrour et al., 2004), using a two-fold change criterion
|
| Growth protocol |
Flies were grown on regular yeast diet, switched to no-yeast food within one hour of eclosion, and yeast-starved for 5 days posteclosion to lower their endogenous JH titer and to synchronize their physiology (cf. Tu and Tatar, 2003; Gershman et al., 2007).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizole/Chloroform extraction
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix Protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix Protocol
|
| Scan protocol |
Standard Affymetrix Protocol
|
| Description |
A study to look at the antagonistic effects of JH and 20E on the expression of antimicrobial peptides (AMPs) in Drosophila.
|
| Data processing |
Expression data were analyzed for significant over- or underrepresentation of gene ontology (GO) terms with the web application FatiGO (Al-Shahrour et al., 2004), using a two-fold change criterion. To test whether JH treatment significantly suppresses expression of AMPs we used t-tests implemented in JMP IN 5.1. (SAS Institute; Sall et al., 2004).
|
| |
|
| Submission date |
Sep 05, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Andreas Heyland |
| E-mail(s) |
[email protected]
|
| URL |
http://www.comparativephys.ca/members/aheyland
|
| Organization name |
University of Guelph
|
| Department |
Integrative Biology
|
| Street address |
50 Stone Rd East
|
| City |
Guelph |
| State/province |
ON |
| ZIP/Postal code |
N1G2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE9001 |
Whole body transcriptional response of female fruitflies to juvenile hormone |
|
