|
| Status |
Public on Sep 12, 2007 |
| Title |
PBMCs-Type 1 Diabetes-IDDM32_T1D_New |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs from Type 1 Diabetes patient and the timepoint at diagnosis.
|
| Organism |
Homo sapiens |
| Characteristics |
Age: 14 yrs, when sample taken.
Gender: F
Ethnicity: Not Hispanic or Latino
Race: White
Illness: Type 1 Diabetes
Treatment: Insulin
pH<7.30 at time of diagnosis: No
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
EukGE-WS2v5_450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 System.
|
| Description |
Type 1 Diabetes patient and the timepoint at diagnosis.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 11, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Ellen Grishman |
| E-mail(s) |
[email protected]
|
| Organization name |
UTSW Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE9006 |
Gene expression in PBMCs from children with diabetes |
|
