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Sample GSM228808 Query DataSets for GSM228808
Status Public on Sep 12, 2007
Title HEL Mlo 2
Sample type RNA
 
Source name HEL-Ig BM cells
Organism Mus musculus
Characteristics Strain C57BL/7
Treatment protocol HEL treated samples were washed after 5 days, replaced with new media with no IL-7, but 1ug/mL HEL. Control samples were treated the same except no HEL in the media. HEL Mlo samples were sorted for IgMa low, IgDneg. HEL 48h samples were sorted for B220+ IgMahi, GFP+ cells after 48h in HEL. BM 48h Ctrl samples were sorted for B220+ IgMahi GFPneg after 48h in culture with no HEL.
Growth protocol 5 day culture in RPMI + 10% FBS, Pen/Strep, L-glut, 2-ME, 16ng/mL IL-7
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy protocol
Label biotin
Label protocol Ambion cDNA synthesis with T7 oligo dT primer (Array Script). Labeling with biotin-11-CTP and biotin-16-UTP.
 
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description LET_101222
Data processing MAS 4
 
Submission date Sep 11, 2007
Last update date Aug 14, 2011
Contact name Lina Tze
E-mail(s) [email protected]
Phone 011-61-2-6125-2031
Organization name John Curtin School of Medical Research
Street address
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
 
Platform ID GPL32
Series (1)
GSE2227 The role of basal immunoglobulin signaling in immature B cell development

Data table header descriptions
ID_REF
VALUE MAS 4 signal

Data table
ID_REF VALUE
AFFX-MurIL10_at 4.295
AFFX-MurIL4_at 1.712
AFFX-MurFAS_at 15.2
AFFX-BioB-5_at 218.4
AFFX-BioB-M_at 509.5
AFFX-BioC-5_at 301.3
AFFX-BioDn-5_at 283.8
AFFX-CreX-5_at 3138
AFFX-CreX-3_at 4545
AFFX-ThrX-5_at 7.595
AFFX-BioB-5_st 4.02
AFFX-BioC-3_st 2.886
AFFX-CreX-5_st 34.64
AFFX-CreX-3_st 35.28
AFFX-b-ActinMur/M12481_5_at 4683
AFFX-b-ActinMur/M12481_M_at 5004
AFFX-GapdhMur/M32599_5_at 4296
AFFX-GapdhMur/M32599_M_at 4134
AFFX-GapdhMur/M32599_3_at 3762
AFFX-TransRecMur/X57349_5_at 60.68

Total number of rows: 12654

Table truncated, full table size 194 Kbytes.




Supplementary file Size Download File type/resource
GSM228808.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM228808.CHP.gz 4.2 Mb (ftp)(http) CHP
GSM228808.EXP.gz 462 b (ftp)(http) EXP
Processed data included within Sample table
Processed data provided as supplementary file

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