|
| Status |
Public on Nov 15, 2007 |
| Title |
2426 aerobic PSY rep_8 |
| Sample type |
RNA |
| |
|
| Source name |
B. japonicum regR mutant strain grown aerobically
|
| Organism |
Bradyrhizobium japonicum |
| Characteristics |
Genotype: 2426
Growth condition: aerobic
Medium: PSY
|
| Treatment protocol |
no treatment was applied
|
| Growth protocol |
cells were grown to an OD600 0.4-0.5 in PSY medium; soybean nodules were harvested 13 and 21 days post inoculation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted as described by Hauser et al. (2007) and Pessi et al.(2007)
|
| Label |
biotin
|
| Label protocol |
2.8 mg (free-living cultures) and 7.8 mg (nodules) cDNA was biotin-labeled
|
| |
|
| Hybridization protocol |
Modification of Affymetrix protocol used for P. aeruginosa
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
B. japonicum regR mutant strain grown aerobically
|
| Data processing |
GeneChip Operating Software (GCOS) version 1.1 (Affymetrix)
|
| |
|
| Submission date |
Sep 12, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Stefan Zoller |
| E-mail(s) |
[email protected]
|
| Organization name |
ETH Zurich
|
| Department |
Genetic Diversity Centre
|
| Street address |
Universitatstrasse 16
|
| City |
Zurich |
| ZIP/Postal code |
8092 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL3401 |
| Series (2) |
| GSE9026 |
New target genes controlled by the Bradyrhizobium japonicum two-component regulatory system RegSR |
| GSE9100 |
Comparison analysis of B. japonicum mutant and wild type grown under different conditions |
|
