|
| Status |
Public on Jul 12, 2017 |
| Title |
M1 Macrophages_Normal_IFNg_4 |
| Sample type |
RNA |
| |
|
| Source name |
Primary monocytes cultured in normal glucose, IFNgamma
|
| Organism |
Homo sapiens |
| Characteristics |
patientno: 4
treatment: IFNg
glucose: NG
cell type: monocyte-derived macrophages
cell subtype: M1
|
| Treatment protocol |
After isolating monocytes from buffy coats, these cells were cultured with IFNg (100ng/ml), IL4 (10ng/ml) and control (without cytokine) for 6 days
|
| Growth protocol |
Primary human monocytes were cultured in serum free medium supplemented by M-CSF (5ng/ml) for 6 days with specific cytokine stimulations in order to differentiate in to different sub-types of macrophage population (M0,M1 and M2).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by using E.Z.N.A. total RNA kit. Extraction procedure was according to E.Z.N.A. total RNA kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Monocytes cultured in normal glucose (5mM) for 6days, IFNgamma (100ng/ml)
Normal_IFNg_4
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of human Hugene-1_0-type from Affymetrix. A Custom CDF Version 19 with Entrez based gene definitions was used to annotate the arrays.The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Aug 31, 2016 |
| Last update date |
Jul 12, 2017 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20171 |
| Series (1) |
| GSE86298 |
Effect of hyperglycemia on the transcriptional profile of primary human macrophages |
|
