|
| Status |
Public on Nov 01, 2007 |
| Title |
Young sedentary subject, biological replicate (YS12) |
| Sample type |
RNA |
| |
|
| Source name |
Vastus Lateralis muscle,young sedentary subject
|
| Organism |
Homo sapiens |
| Characteristics |
no additional information
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNAs were extracted using QIAGEN'S RNeasy Fibrous Tissue Kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the The GeneChip® Scanner 3000 7G .
|
| Description |
Expression of mRNA in Vastus Lateralis muscle,young sedentary subject
|
| Data processing |
The data were analyzed with dChip using quantile normalization and PM-only Model Based Expression Indexing.
|
| |
|
| Submission date |
Sep 19, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yan W. Asmann |
| E-mail(s) |
[email protected]
|
| Organization name |
Mayo Clinic
|
| Street address |
4500 San Pablo Road South
|
| City |
Jacksonville |
| State/province |
FL |
| ZIP/Postal code |
32224 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9103 |
Skeletal Muscle Transcript Profiles in Trained or Sedentary Young and Old Subjects |
|
| Relations |
| Reanalyzed by |
GSE119087 |
