|
| Status |
Public on Mar 07, 2017 |
| Title |
NC Tissue mix 1 |
| Sample type |
RNA |
| |
|
| Source name |
normal tissue from Balb/c mouse, 5 weeks of age
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mixture of mouse normal tissues
|
| Treatment protocol |
No treatment was provided.
|
| Growth protocol |
The tumor was grown subcutaneously in Balb/c nude mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
|
| Description |
Gene expression data from mouse normal tissues
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 07, 2016 |
| Last update date |
Mar 07, 2017 |
| Contact name |
Takuro Nakamura |
| E-mail(s) |
[email protected]
|
| Phone |
81-3-3570-0462
|
| Organization name |
Japanese Foundation for Cancer Research
|
| Department |
The Cancer Institute
|
| Lab |
Carcinogenesis
|
| Street address |
3-8-31 Ariake, Koto-ku
|
| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE86501 |
Gene expression profiles of mouse alveolar soft part sarcoma |
| GSE86502 |
Gene expression profiles of the mouse model for alveolar soft part sarcoma |
|
