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Sample GSM230663 Query DataSets for GSM230663
Status Public on Jan 02, 2008
Title High selected line MA (2525.H1)
Sample type RNA
 
Source name Wing discs MA H line
Organism Drosophila melanogaster
Characteristics Original Massachussetts high angular offset
Age: 3rd instar larvae median age 6.2 hr prior to pupariation.
Treatment protocol Larvae were dissected in 30 μl drops of cold PBS on siliconized slides, with needle-tip tweezers where wing disks were rapidly excised and cleaned with tungsten wire needles. Each pair of disks was then transferred from the needle tip to the bottom of a 0.5 ml microcentrifuge tube in dry ice, where 100 disks were collected in single microcentrifuge tubes.
Growth protocol Lines H and L were derived from a wild Massachusetts population sample by 20 generations of divergent selection, then isogenized. H and L were then crossed to make hybrid populations A-D. After 34 generations of genomic mingling, hybrid lines were divergently selected for 25 generations, then inbred for 10. The California lines were selected from a separate population. Flies were cultured at 20ºC on cornmeal agar medium.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from wing discs.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 2500.
 
Submission date Sep 20, 2007
Last update date Aug 28, 2018
Contact name Ralph J Greenspan
E-mail(s) [email protected]
Phone 858-626-2075
Organization name The Neurosciences Institute
Street address 10640 John Jay Hopkins Dr.
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL1322
Series (1)
GSE9107 Expression data of Drosophila 3rd instar larval wing discs taken from strains selected for wing shape.
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 1604.5 P
AFFX-BioB-M_at 2096.8 P
AFFX-BioB-3_at 1398.3 P
AFFX-BioC-5_at 3996.7 P
AFFX-BioC-3_at 3639 P
AFFX-BioDn-5_at 8233.2 P
AFFX-BioDn-3_at 26432.5 P
AFFX-CreX-5_at 63100.5 P
AFFX-CreX-3_at 79007.8 P
AFFX-DapX-5_at 9.4 A
AFFX-DapX-M_at 232.2 A
AFFX-DapX-3_at 90 A
AFFX-LysX-5_at 12.7 A
AFFX-LysX-M_at 63 A
AFFX-LysX-3_at 248.4 P
AFFX-PheX-5_at 13.7 A
AFFX-PheX-M_at 39 A
AFFX-PheX-3_at 63.6 A
AFFX-ThrX-5_at 202.5 A
AFFX-ThrX-M_at 71.8 A

Total number of rows: 18952

Table truncated, full table size 360 Kbytes.




Supplementary file Size Download File type/resource
GSM230663.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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