|
| Status |
Public on Jan 02, 2008 |
| Title |
Low selected line MA (2529.L2) |
| Sample type |
RNA |
| |
|
| Source name |
Wing discs MA L line
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Original Massachussetts low angular offset
Age: 3rd instar larvae median age 6.2 hr prior to pupariation.
|
| Treatment protocol |
Larvae were dissected in 30 μl drops of cold PBS on siliconized slides, with needle-tip tweezers where wing disks were rapidly excised and cleaned with tungsten wire needles. Each pair of disks was then transferred from the needle tip to the bottom of a 0.5 ml microcentrifuge tube in dry ice, where 100 disks were collected in single microcentrifuge tubes.
|
| Growth protocol |
Lines H and L were derived from a wild Massachusetts population sample by 20 generations of divergent selection, then isogenized. H and L were then crossed to make hybrid populations A-D. After 34 generations of genomic mingling, hybrid lines were divergently selected for 25 generations, then inbred for 10. The California lines were selected from a separate population. Flies were cultured at 20ºC on cornmeal agar medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from wing discs.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 2500.
|
| |
|
| Submission date |
Sep 20, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ralph J Greenspan |
| E-mail(s) |
[email protected]
|
| Phone |
858-626-2075
|
| Organization name |
The Neurosciences Institute
|
| Street address |
10640 John Jay Hopkins Dr.
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE9107 |
Expression data of Drosophila 3rd instar larval wing discs taken from strains selected for wing shape. |
|
| Relations |
| Reanalyzed by |
GSE119084 |
