|
| Status |
Public on Sep 22, 2016 |
| Title |
Before pre-differentiation, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic stem cell aggregates
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Cell aggregates
|
| Treatment protocol |
On day 7, medium was changed to pre-differentiation protocol (with BMP4 and bFGF) for 3 days.
|
| Growth protocol |
Cell aggregates cultured in ES complete medium for 7 days (with LIF)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Plus Mini Kit (Qiagen) following the manufacture’s instruction
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Herring sperm DNA, Acetylated BSA (50mg/mL), Eukaryotic Hybridization Controls (Affymetrix) •Heat 65oC for 5 minutes, 99oC for 5 minutes, 45oC for 5 minutes centrifuge top speed for 5 minutes •Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000 7G using AGCC (Affymetrix GeneChip Command Console)
|
| Description |
Gene expression data
|
| Data processing |
The data were analyzed with Affymetrix® Transcriptome Analysis Console (TAC) Softwareusing Affymetrix default analysis settings
|
| |
|
| Submission date |
Sep 19, 2016 |
| Last update date |
Sep 22, 2016 |
| Contact name |
Shuting zhao |
| E-mail(s) |
[email protected]
|
| Organization name |
The ohio state university
|
| Street address |
Room 340H, 460 West 12th Ave., Biomedical research
|
| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE87066 |
Expression data from cell aggregates before and after pre-differentiation |
|
