|
| Status |
Public on Oct 22, 2016 |
| Title |
121002-lung carcinoma tissue |
| Sample type |
RNA |
| |
|
| Source name |
lung carcinoma tissue from 121002
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: lung cancer
tissue: lung carcinoma tissue
gender: female
geographic location: Xuanwei County, China
patient id: 121002
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine's linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized in an Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2x SSC at 42℃ for 5 min, and 0.2x SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
Gene expression of 121002-lung carcinoma tissue.
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and GeneSpring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Oct 21, 2016 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Yanliang Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
First Affiliated Hospital of Kunming Medical University
|
| Department |
Department of Clinical Laboratory
|
| Lab |
Yunnan Key Laboratory of Laboratory Medicine
|
| Street address |
No. 295, Xichang Road
|
| City |
Kunming |
| State/province |
Yunnan province |
| ZIP/Postal code |
650032 |
| Country |
China |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE89039 |
Gene expression profiling in 8 lung cancer in Xuanwei samples |
| GSE89047 |
Gene expression profiling and copy number alterations in 8 lung cancer in Xuanwei samples |
|
| Relations |
| Reanalyzed by |
GSE113533 |
