|
| Status |
Public on Aug 01, 2020 |
| Title |
6 hr 50 mg/kg CYC065 treatment-tumors from the Th-MYCN GEMM (T17) |
| Sample type |
RNA |
| |
|
| Source name |
6 hr CYC065 50 mg/kg treatment-tumors from the Th-MYCN GEMM
|
| Organism |
Mus musculus |
| Characteristics |
tissue: NB tumors
treatment: 6 hr CYC065 50 mg/kg
|
| Treatment protocol |
Kelly, BE(2)C AND SH-SY-5Y were treated with 1 µM CYC065 for 1 hour and SKNAS was treated with 5 µM CYC065 for 1 hour. Mice were treated with CYC065 50 mg/kg or vehicle for 6 hours.
|
| Growth protocol |
Kelly, SKNAS, BE(2)C AND SH-SY-5Y cell lines were maintained in DMEM as recommended by the suppliers, supplemented with 10% fetal calf serum (FCS) (Gibco), and were maintained at 37°C under 5% CO2 in air.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells or tissue were harvested using Qiagen mRNeasy Plus Kit
|
| Label |
biotin
|
| Label protocol |
cRNA were prepared according to the standard Affymetrix GeneChip® WT PLUS Reagent Kit protocol from 100 ng total RNA and labeled (Gene Chip® human or mouse transcriptome expression array)
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of ss c-DNA were hybridized for 16 hr at 45C on Gene Chip® human or mouse transcriptome expression array. GeneChips were washed and stained in the Gene Chip® Fluidics station 450
|
| Scan protocol |
GeneChips were scanned using the Gene Chip® Scanner
|
| Data processing |
Samples were RMA normalized using the “limma” package from R and differentially expressed genes were called using a linear model and empirical Bayes statistics from the “affy” package
|
| |
|
| Submission date |
Oct 26, 2016 |
| Last update date |
Aug 01, 2020 |
| Contact name |
Evon Poon |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Cancer Research
|
| Department |
Clinical Studies
|
| Lab |
Evon Poon
|
| Street address |
15, Cotswold Road, Godstone Road
|
| City |
Belmont |
| State/province |
Surrey |
| ZIP/Postal code |
SM2 5NG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE89197 |
Blockade of MYCN-dependent transcription using orally-bioavailable CDK9 inhibitors |
|
