|
| Status |
Public on Dec 28, 2007 |
| Title |
WT_totalRNA_1_F |
| Sample type |
RNA |
| |
|
| Source name |
whole plant tissue germinated and grown for ~6 days on 8 uM of 17beta-estradiol (Sigma), added from the 8 mM stock in DMSO
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Contains empty vector
|
| Treatment protocol |
DMSO
|
| Growth protocol |
plant tissue germinated and grown for ~6 days on DMSO under 16 hours light, 8 hours dark at 25 degrees C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from whole plant tissues using TRIzol reagent following instructions from the manufacturer (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Fifteen ug of total RNA was used to synthesize double-stranded cDNA using the GeneChip One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was generated by in vitro transcription using the GeneChip IVT Labeling Kit (Affymetrix),
|
| |
|
| Hybridization protocol |
Whole Genome Tiling Array was hybridized for 16 hrs, washed and stained with a streptavidin-phycoerythrin conjugate on the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned on Affymetrix Scanner G7
|
| Description |
Empty vector control for estradiol-specific effects
|
| Data processing |
Gene chip data files were processed usingTileMap.
|
| |
|
| Submission date |
Oct 13, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Joseph R. Ecker |
| E-mail(s) |
[email protected]
|
| Phone |
1-858-453-4100 x 1975
|
| URL |
http://signal.salk.edu/
|
| Organization name |
The Salk Institute
|
| Department |
Genomic Analysis Laboratory
|
| Lab |
Joseph R. Ecker
|
| Street address |
10010 N. Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL1979 |
| Series (1) |
| GSE9317 |
Genome-Wide High-Resolution Mapping of Exosome Substrates Reveals Hidden Features in the Arabidopsis Transcriptome |
|
