|
| Status |
Public on Oct 22, 2007 |
| Title |
striatum_Hdh4Q80_9S |
| Sample type |
RNA |
| |
|
| Source name |
Striatum (brain)
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6; Gender: female; Age: 12 months; Tissue: striatum (brain); Mouse model: Hdh4/Q80 transgenic mice (Shelbourne et al, Hum Mol Genet 8:763-774,1999)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol (Invitrogen) followed by RNeasy column cleanup (Qiagen) using the manufacturers’ protocols
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip® IVT Labeling Kit, according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual)
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol (GeneChip® Expression Analysis Technical Manual)
|
| Scan protocol |
Affymetrix® GeneChip® Scanner 3000 with GCOS software according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual)
|
| Description |
Striatal sample from a 12-month-old Hdh4/Q80 mouse.
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
Oct 19, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Alexandre Kuhn |
| E-mail(s) |
[email protected]
|
| Organization name |
NIH
|
| Department |
NIA
|
| Lab |
Laboratory of Neurogenetics
|
| Street address |
35 Convent Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-3707 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (2) |
| GSE9375 |
Striatal gene expression data from 12 months-old Hdh4/Q80 mice and control mice. |
| GSE10263 |
Mutant huntingtin's effects on striatal gene expression in mice |
|
