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Sample GSM239384 Query DataSets for GSM239384
Status Public on Sep 22, 2008
Title vastus lateralis, protein intake: 1.00 g/kg, young, subject 1
Sample type RNA
 
Source name vastus lateralis, protein intake: 1.00 g/kg, young
Organism Homo sapiens
Characteristics Protein intake: 1.00 g/kg
Trial: P125
Sex: Male
Age: Y (21-43 y)
Treatment protocol Three randomized controlled feeding trials with protein intakes of 63% (P63: 0.50 g/kg-1/d-1), 94% (P94: 0.75 g/kg-1/d-1), and 125% (P125: 1.00 g/kg-1/d-1) of the recommended dietary allowance (RDA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the frozen muscle sample using Tri-Reagent following the manufacturerÆs instructions. Isolated total RNA was further purified using RNeasy Mini Kits following manufacturerÆs protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from a minimum of 5 ug total RNA
 
Hybridization protocol Affymetrix protocol
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
Description Gene expression for younger male following two weeks of dietary protein intake 1.00 g/kg
Data processing The raw data were summarized using Affymetrix Microarray Suite (MAS) 5.0 software
 
Submission date Oct 24, 2007
Last update date Aug 28, 2018
Contact name Wayne Campbell
E-mail(s) [email protected]
Phone 765-494-8236
Fax 765-494-0674
Organization name Purdue University
Department Foods and Nutrition
Street address 700 West State Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
 
Platform ID GPL570
Series (1)
GSE9419 The skeletal muscle transcript profile reflects responses to inadequate protein intake in younger and older males
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 240.7 M 0.05447
1053_at 101.1 P 0.043968
117_at 26.8 A 0.267463
121_at 391.7 P 0.011447
1255_g_at 24.2 A 0.250724
1294_at 59.7 A 0.204022
1316_at 56.8 A 0.11716
1320_at 11.8 A 0.849473
1405_i_at 13.9 A 0.586954
1431_at 16.9 A 0.418069
1438_at 13.1 A 0.849473
1487_at 631 P 0.014937
1494_f_at 98 A 0.162935
1552256_a_at 29 A 0.466064
1552257_a_at 96 A 0.080566
1552258_at 70.3 A 0.129639
1552261_at 30.7 A 0.171387
1552263_at 3.5 A 0.753906
1552264_a_at 51.7 A 0.067627
1552266_at 44.1 A 0.303711

Total number of rows: 54675

Table truncated, full table size 1412 Kbytes.




Supplementary file Size Download File type/resource
GSM239384.CEL.gz 5.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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