|
| Status |
Public on Sep 22, 2008 |
| Title |
vastus lateralis, protein intake:0.50 g/kg, old, subject 22 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis, protein intake:0.50 g/kg, old
|
| Organism |
Homo sapiens |
| Characteristics |
Protein intake:0.50 g/kg
Trial:P63
Sex: Male
Age: O (63-79 y)
|
| Treatment protocol |
Three randomized controlled feeding trials with protein intakes of 63% (P63: 0.50 g/kg-1/d-1), 94% (P94: 0.75 g/kg-1/d-1), and 125% (P125: 1.00 g/kg-1/d-1) of the recommended dietary allowance (RDA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the frozen muscle sample using Tri-Reagent following the manufacturerÆs instructions. Isolated total RNA was further purified using RNeasy Mini Kits following manufacturerÆs protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from a minimum of 5 ug total RNA
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
|
| Description |
Gene expression for older male following two weeks of dietary protein intake of 0.50 g/kg
|
| Data processing |
The raw data were summarized using Affymetrix Microarray Suite (MAS) 5.0 software
|
| |
|
| Submission date |
Oct 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Wayne Campbell |
| E-mail(s) |
[email protected]
|
| Phone |
765-494-8236
|
| Fax |
765-494-0674
|
| Organization name |
Purdue University
|
| Department |
Foods and Nutrition
|
| Street address |
700 West State Street
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9419 |
The skeletal muscle transcript profile reflects responses to inadequate protein intake in younger and older males |
|
| Relations |
| Reanalyzed by |
GSE119087 |
