|
| Status |
Public on Jun 19, 2018 |
| Title |
listeria infected DmSTING RNAi flies, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
whole flies, DmSTING RNAi, listeria inoculated
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: DmSTING RNAi flies
tissue: whole adult flies
pathogen: listeria monocytogenes
hours post-infection: 24
|
| Treatment protocol |
Intrathoracic injection with 23 nl of 10e7 CFU/ml listeria using Drummond scientific nanoject II
|
| Growth protocol |
Flies were raised on standard cornmeal at 25C and 65% relative humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were homogenized and total RNA was extracted using Thermo GeneJet RNA purification kit. RNA QC was performed using Advanced Analytical Technology Fragment Analyzer
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip 3' IVT Express Kit
|
| |
|
| Hybridization protocol |
Affynetrix GeneChip Hybridization, Wash, and Stain Kit, Fluidics Station 450 (fluidics Midi_euk3 v3) and Hybridization Oven 640
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data following inoculation'
|
| Data processing |
GeneSpring GX 14.5 using the default summarization/scaling/normalization algorithms.
|
| |
|
| Submission date |
Nov 22, 2016 |
| Last update date |
Jun 19, 2018 |
| Contact name |
Alan G. Goodman |
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
100 Dairy Rd., BLS 202
|
| City |
Pullman |
| State/province |
WASHINGTON |
| ZIP/Postal code |
99164 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE85473 |
Role of DmSTING in fly |
| GSE90442 |
Whole fly expression data following DmSTING knockdown and listeria infection |
|
