|
| Status |
Public on Dec 01, 2021 |
| Title |
eDRG_3HPI_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Spot cultures were axotomized with a blade on DIV 7 then collected 3 hours post injury
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD-1
developmental stage: Embryonic day 13
tissue: Dorsal Root Ganglia
|
| Treatment protocol |
Spot cultures were axotomized with a blade on DIV 7 then collected 3 hours post injury
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from samples using the miRNeasy Micro Kit as per the manufacturers protocol. RNA was quantified using a Synergy H1 Hybrid Reader and quality was analyzed using an Agilent Bioanalyzer 2100.
|
| Label |
biotin
|
| Label protocol |
Probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
500 ng of RNA was hybridized for 16 hours at 60 rpm at 48°C on Affymetrix GeneChip miRNA 4.0 Array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner G3000 7G
|
| Description |
24052
|
| Data processing |
The data were analyzed with Affymetrix Expression Console (v1.4) using default analysis settings. The signal values were background-corrected with Robust Multi-Array Averaging (RMA) method, quanitle-normalized, and median-polish summarized.
|
| |
|
| Submission date |
Nov 23, 2016 |
| Last update date |
Dec 01, 2021 |
| Contact name |
Valeria Cavalli |
| E-mail(s) |
[email protected]
|
| Organization name |
Washington University in St. Louis
|
| Department |
Anatomy and Neurobiology
|
| Street address |
660 S. Euclid Ave.
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (2) |
| GSE90475 |
miRNA-9 regulates axon regeneration through the transcriptional regulator REST and the epigenetic factor UHRF1 [miRNA array] |
| GSE90477 |
Gene repression by DNA methylation promotes axon regeneration |
|
