Total RNA was extracted from caryopsis using the Trizol method (Invitrogen) and purified with RNeasy MiniKit (Qiagen). cDNA was synthesized and purified with one-cycle cDNA Synthesis Kit (Affymetrix) and GeneChip Sample Cleanup Module (Affymetrix), respectively.
Label
Biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol with GeneChip IVT Labeling Kit and Genechip Sample Cleanup Module (Affymetrix)
Hybridization protocol
Affymetrix standard protocol
Scan protocol
Affymetrix standard protocol
Description
Gene expression data of wild type Zhonghua11 caryopsis, 7 DAF
Data processing
The data were analyzed with Affymetrix GeneChip® Operating Software (GCOS, Version 1.4) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
